Head and neck cancers, especially laryngeal carcinoma, are still an essential therapeutic problem worldwide. The most common risk factors which participate in initiation of cancer progression are tobacco smoking and alcohol abuse. The fundamental factor which confines effective therapy is tumor advanced stage at the moment of diagnosis. For proper determination of cancer progression there still is a need to identify new markers which can describe cancer cells and are also related in invasiveness and tumor matrix rebuild. The studies were performed on tissue samples divided in two groups: from patient who smoked less and above 20 cigarettes a day. We observed the expression of studied antigens as follows: MMP-2 in 100%, TGF-beta1 in 86% and TGF-betaRI in 79% cases. In tumor stroma we observed higher expression of MMP-2, TGF-beta1 in patients with higher exposure to tobacco smoke. Moreover in patients who smoked above 20 cigarettes, we detected higher expression of MMP-2 in tumor stroma (86% vs. 12.5%), compared with expression in cancer cells (3% vs. 3.25%). Expression of TGF-betaRI, in group I, was respectively higher in tumor stroma (16.8%) compared with cancer cells (6%). However we've found no expression of TGF-BRI in samples from patients smoking more then 20 cigarettes a day. In summary we may suggest, that the level of exposure on tobacco smoke play important role on changes in tumor markers expression.