PGC-1β regulates angiogenesis in skeletal muscle

Am J Physiol Endocrinol Metab. 2011 Jul;301(1):E155-63. doi: 10.1152/ajpendo.00681.2010. Epub 2011 Mar 1.

Abstract

Aerobic metabolism requires oxygen and carbon sources brought to tissues via the vasculature. Metabolically active tissues such as skeletal muscle can regulate blood vessel density to match metabolic needs; however, the molecular cues that coordinate these processes remain poorly understood. Here we report that the transcriptional coactivator peroxisome proliferator-activated receptor-γ coactivator-1β (PGC-1β), a potent regulator of mitochondrial biology, induces angiogenesis in skeletal muscle. PGC-1β induces the expression of vascular endothelial growth factor (VEGF) in cell culture and in vivo. The induction of VEGF by PGC-1β requires coactivation of the orphan nuclear receptor estrogen-related receptor-α (ERRα) and is independent of the hypoxia-inducible factor (HIF) pathway. In coculture experiments, overexpression of PGC-1β in skeletal myotubes increases the migration of adjacent endothelial cells, and this depends on VEGF signaling. Transgenic expression of PGC-1β in skeletal myocytes dramatically increases muscular vessel density. Taken together, these data indicate that PGC-1β is a potent regulator of angiogenesis, thus providing a novel link between the regulations of oxidative metabolism and vascular density.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Carrier Proteins / physiology*
  • Cell Movement / genetics
  • Cell Movement / physiology
  • Cells, Cultured
  • ERRalpha Estrogen-Related Receptor
  • Endothelial Cells / metabolism
  • Endothelial Cells / physiology
  • Gene Expression Regulation / genetics
  • Humans
  • Hypoxia-Inducible Factor 1, alpha Subunit / genetics
  • Hypoxia-Inducible Factor 1, alpha Subunit / physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Muscle Fibers, Skeletal / metabolism
  • Muscle Fibers, Skeletal / physiology
  • Muscle, Skeletal / blood supply*
  • Muscle, Skeletal / metabolism*
  • Neovascularization, Physiologic / genetics*
  • RNA-Binding Proteins
  • Receptors, Estrogen / genetics
  • Receptors, Estrogen / physiology
  • Vascular Endothelial Growth Factor A / genetics
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • Carrier Proteins
  • HIF1A protein, human
  • Hypoxia-Inducible Factor 1, alpha Subunit
  • PPARGC1B protein, human
  • RNA-Binding Proteins
  • Receptors, Estrogen
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A