Evolutionary sequence of cytogenetic aberrations during the oncogenesis of plasma cell disorders. Direct evidence at single cell level

Leuk Res. 2011 Aug;35(8):1114-6. doi: 10.1016/j.leukres.2011.02.010. Epub 2011 Mar 5.

Abstract

Bone marrow specimens from 185 patients with plasma cell disorders (PCD) were investigated by fluorescence in situ hybridization (FISH) in order to determine the temporal sequence of cytogenetic aberrations. In 25 cases combined FISH analysis has also been performed at single cell level. Clonal evolution was observed in 16% of cases. The Δ13 was preceded by t(4;14)(p16;q32) and t(14;16)(q32;q23) translocations. Deletion of p53 gene was a secondary aberration compared to Δ13 and t(11;14)(q13;q32) translocation. In 22% of all cases with recurrent IGH translocation, this aberration was presented only in a subset of purified plasma cells questioning its initiating role.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Marrow / metabolism
  • Bone Marrow / pathology
  • Chromosome Aberrations*
  • Chromosome Disorders*
  • Chromosomes, Human, Pair 11 / genetics
  • Chromosomes, Human, Pair 13 / genetics
  • Chromosomes, Human, Pair 14 / genetics
  • Chromosomes, Human, Pair 16 / genetics
  • Chromosomes, Human, Pair 4 / genetics
  • Evolution, Molecular*
  • Humans
  • Immunoglobulin Heavy Chains / genetics*
  • In Situ Hybridization, Fluorescence
  • Leukemia, Plasma Cell / genetics*
  • Leukemia, Plasma Cell / pathology
  • Multiple Myeloma / genetics*
  • Multiple Myeloma / pathology
  • Translocation, Genetic / genetics

Substances

  • Immunoglobulin Heavy Chains