A new device for rapid isolation by size and characterization of rare circulating tumor cells

Anticancer Res. 2011 Feb;31(2):427-41.

Abstract

Background: Circulating tumor cells (CTCs) likely derive from clones in the primary tumor, suggesting that they can be used for all biological tests applying to the primary cells.

Materials and methods: The ScreenCell® devices are single-use and low-cost innovative devices that use a filter to isolate and sort tumor cells by size.

Results: The ScreenCell® Cyto device is able to isolate rare, fixed, tumor cells, with a high recovery rate. Cells are well preserved morphologically. Immunocytochemistry and FISH assays can be performed directly on the filter. The ScreenCell® CC device allows isolation of live cells able to grow in culture. High quality genetic materials can be obtained directly from tumor cells isolated on the ScreenCell® MB device filter.

Conclusion: Due to their reduced size, versatility, and capacity to isolate CTCs within minutes, the ScreenCell® devices may be able to simplify and improve non-invasive access to tumor cells.

MeSH terms

  • Carcinoma, Non-Small-Cell Lung / blood
  • Carcinoma, Non-Small-Cell Lung / genetics
  • Carcinoma, Non-Small-Cell Lung / pathology
  • Cell Line, Tumor
  • Cell Separation / instrumentation
  • Cell Separation / methods
  • Cell Size
  • Colorectal Neoplasms / blood
  • Colorectal Neoplasms / genetics
  • Colorectal Neoplasms / pathology
  • Cytological Techniques / instrumentation*
  • Cytological Techniques / methods*
  • ErbB Receptors / genetics
  • Exons
  • Filtration / instrumentation
  • Filtration / methods
  • Gene Deletion
  • HT29 Cells
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Lung Neoplasms / blood
  • Lung Neoplasms / genetics
  • Lung Neoplasms / pathology
  • Neoplastic Cells, Circulating / pathology*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • ErbB Receptors