Urinary transforming growth factor-β1 in children with obstructive uropathy

Nephrology (Carlton). 2011 Aug;16(6):595-8. doi: 10.1111/j.1440-1797.2011.01459.x.

Abstract

Aim: Obstructive uropathies (OU) in childhood constitute one of the major causes of chronic renal insufficiency. Transforming growth factor-β1 (TGF-β1) is considered to be the major fibrogenic growth factor. The aim of the present study was to investigate urinary TGF-β1 levels in children with obstructive and non-obstructive uropathies (NOU).

Methods: This study involved 19 children with OU, 11 children with non-obstructive hydronephrosis and 21 healthy children. Urinary TGF-β1, proteinuria, microalbuminuria and urinary α1-microglobulin were measured, and renal function was assessed. The results were statistically analyzed.

Results: Mean urinary TGF-β1 concentrations in patients with OU were significantly higher than those with NOU (4.14 ± 0.67 creatinine vs 1.80 ± 0.24 pg/mmol creatinine, P < 0.05) and healthy controls (1.66 ± 0.28 pg/mmol creatinine, P < 0.05). Positive correlations of urinary TGF-β1 concentrations with proteinuria (r = 0.87, P < 0.0001) and urinary α1-microglobulin (r = 0.82, P = 0.0002) were found in patients with OU.

Conclusion: Children with OU have higher urinary TGF-β1 than children with NOU. Urinary TGF-β1 may be a useful non-invasive tool for the differential diagnosis between OU and NOU in children. A positive correlation of TGF-β1 with markers of renal tissue damage in patients with OU was found.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Albuminuria / etiology
  • Albuminuria / urine
  • Alpha-Globulins / urine
  • Analysis of Variance
  • Biomarkers / urine
  • Case-Control Studies
  • Child, Preschool
  • Creatinine / urine
  • Cross-Sectional Studies
  • Czech Republic
  • Female
  • Glomerular Filtration Rate
  • Humans
  • Hydronephrosis / etiology
  • Hydronephrosis / urine
  • Infant
  • Kidney / physiopathology
  • Male
  • Predictive Value of Tests
  • Renal Insufficiency, Chronic / diagnosis
  • Renal Insufficiency, Chronic / etiology*
  • Renal Insufficiency, Chronic / physiopathology
  • Renal Insufficiency, Chronic / urine
  • Transforming Growth Factor beta1 / urine*
  • Up-Regulation
  • Ureteral Obstruction / complications
  • Ureteral Obstruction / diagnosis
  • Ureteral Obstruction / physiopathology
  • Ureteral Obstruction / urine*
  • Urethral Obstruction / complications
  • Urethral Obstruction / diagnosis
  • Urethral Obstruction / physiopathology
  • Urethral Obstruction / urine*

Substances

  • Alpha-Globulins
  • Biomarkers
  • TGFB1 protein, human
  • Transforming Growth Factor beta1
  • alpha-1-microglobulin
  • Creatinine