Tissue specificity of Caenorhabditis elegans enhanced RNA interference mutants

Genetics. 2011 May;188(1):235-7. doi: 10.1534/genetics.111.127209. Epub 2011 Mar 8.

Abstract

Gene knockdown by RNA interference (RNAi) in Caenorhabditis elegans is readily achieved by feeding bacteria expressing double-stranded RNA (dsRNA). Enhanced RNAi (Eri) mutants facilitate RNAi due to their hypersensitivity to dsRNA. Here, we compare eight Eri mutants for sensitivity to ingested dsRNA, targeting a variety of tissue-specific genes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics*
  • Genetic Testing
  • Mutation / genetics*
  • Organ Specificity / genetics*
  • Phenotype
  • RNA Interference*
  • RNA, Double-Stranded / metabolism

Substances

  • RNA, Double-Stranded