Abstract
A critical issue for current liposomal carriers in clinical applications is their leakage of the encapsulated drugs that are cytotoxic to non-target tissues. We have developed partially polymerized liposomes composed of polydiacetylene lipids and saturated lipids. Cross-linking of the diacetylene lipids prevents the drug leakage even at 40 °C for days. These inactivated drug carriers are non-cytotoxic. Significantly, more than 70% of the encapsulated drug can be instantaneously released by a laser that matches the plasmon resonance of the tethered gold nanoparticles on the liposomes, and the therapeutic effect was observed in cancer cells. The remote activation feature of this novel drug delivery system allows for precise temporal and spatial control of drug release.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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1,2-Dipalmitoylphosphatidylcholine / chemistry
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Aniline Compounds
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Biological Availability
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Breast Neoplasms / drug therapy
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Cell Line, Tumor
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Cell Survival / drug effects
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Cryoelectron Microscopy
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Delayed-Action Preparations / chemical synthesis
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Delayed-Action Preparations / chemistry*
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Delayed-Action Preparations / radiation effects
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Diynes / chemistry
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Doxorubicin / administration & dosage
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Doxorubicin / pharmacology
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Drug Stability
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Endocytosis
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Female
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Fluoresceins / administration & dosage
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Fluoresceins / pharmacokinetics
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Glycine
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Gold / chemistry
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Humans
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Imino Acids / administration & dosage
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Imino Acids / pharmacokinetics
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Lasers
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Liposomes / chemical synthesis
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Liposomes / chemistry*
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Liposomes / radiation effects
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Lysophospholipids / chemistry
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Metal Nanoparticles / chemistry*
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Metal Nanoparticles / radiation effects
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Organotechnetium Compounds / administration & dosage
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Organotechnetium Compounds / pharmacokinetics
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Particle Size
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Phosphatidylcholines / chemistry
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Phosphatidylethanolamines / chemistry
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Polymers / chemical synthesis
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Polymers / chemistry
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Surface Plasmon Resonance
Substances
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Aniline Compounds
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Delayed-Action Preparations
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Diynes
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Fluoresceins
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Imino Acids
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Liposomes
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Lysophospholipids
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Organotechnetium Compounds
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Phosphatidylcholines
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Phosphatidylethanolamines
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Polymers
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1,2-Dipalmitoylphosphatidylcholine
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Gold
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1,2-bis(10,12-tricosadiynoyl)phosphatidylcholine
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Doxorubicin
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technetium Tc 99m mebrofenin
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Glycine
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fluorexon