Abstract
The adenovirus E1a protein stimulates transcription of viral early genes. Recent experiments indicate that E1a contains a transcriptional activating region, which functions when directed to a promoter. Because E1a is not a sequence-specific DNA binding protein, how it targets to viral promoters has been a question. Several of the viral early promoters contain one or more binding sites for ATFs, a family of cellular transcription factors. Here we show that E1a can function through a specific ATF protein, designated ATF-2. We provide evidence that E1a interacts with a discrete region of promoter-bound ATF-2, thereby positioning the E1a activating region at a viral promoter.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Activating Transcription Factors
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Adenovirus Early Proteins
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Animals
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Base Sequence
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Binding Sites
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Blood Proteins / genetics
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Blood Proteins / metabolism*
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Cell Line
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Cloning, Molecular
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DNA-Binding Proteins / metabolism
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Gene Expression Regulation, Viral*
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Humans
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Models, Genetic
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Molecular Sequence Data
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Mutation
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Neoplasm Proteins / metabolism
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Oligonucleotide Probes
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Oncogene Proteins, Viral / genetics
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Oncogene Proteins, Viral / metabolism*
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Promoter Regions, Genetic
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RNA, Messenger / genetics
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae Proteins*
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Trans-Activators / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic*
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Transfection
Substances
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Activating Transcription Factors
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Adenovirus Early Proteins
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Blood Proteins
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DNA-Binding Proteins
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Fungal Proteins
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GAL4 protein, S cerevisiae
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Neoplasm Proteins
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Oligonucleotide Probes
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Oncogene Proteins, Viral
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RNA, Messenger
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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Transcription Factors