Artificial leucine rich repeats as new scaffolds for protein design

Bioorg Med Chem Lett. 2011 Apr 15;21(8):2372-5. doi: 10.1016/j.bmcl.2011.02.093. Epub 2011 Mar 21.

Abstract

The leucine rich repeat (LRR) motif that participates in many biomolecular recognition events in cells was suggested as a general scaffold for producing artificial receptors. We describe here the design and first total chemical synthesis of small LRR proteins, and their structural analysis. When evaluating the tertiary structure as a function of different number of repeating units (1-3), we were able to find that the 3-repeats sequence, containing 90 amino acids, folds into the expected structure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / metabolism
  • Circular Dichroism
  • Leucine / chemistry*
  • Membrane Proteins / chemistry
  • Membrane Proteins / metabolism
  • Molecular Sequence Data
  • Protein Engineering
  • Protein Structure, Secondary
  • Proteins / chemistry*
  • Proteins / metabolism

Substances

  • Bacterial Proteins
  • Membrane Proteins
  • Proteins
  • inlB protein, Listeria monocytogenes
  • Leucine