Synthesis, structural characterization, formation constants and in vitro cytotoxicity of phenanthroline and imidazolidine-2-thione copper(II) complexes

J Inorg Biochem. 2011 Mar;105(3):329-38. doi: 10.1016/j.jinorgbio.2010.11.017. Epub 2010 Nov 30.

Abstract

The synthesis, crystal structures, physicochemical properties and complex formation constants of [Cu(phen)(2)(L)](ClO(4))(2) complexes, where phen is 1,10-ortho-phenanthroline and L is a series of substituted imidazolidine-2-thione, have been studied. Single crystal X-ray diffraction revealed a distorted trigonal-bipyramidal geometry for all the molecules. The complex formation constants were determined in nonaqueous media by spectrophotometric measurements. Testing copper(II) complexes in mouse neuroblastoma N2a cells persistently infected with the 22L strain of the scrapie prion protein (22L-N2a) resulted in high cytotoxicity but no antiprion activity at nontoxic doses.

MeSH terms

  • Animals
  • Cations, Divalent / chemistry
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Copper / chemistry*
  • Cytotoxicity Tests, Immunologic
  • Ethylenethiourea
  • Imidazolidines / chemistry*
  • Mice
  • Neuroblastoma / metabolism
  • Neuroblastoma / pathology
  • Organometallic Compounds / chemical synthesis*
  • Organometallic Compounds / chemistry
  • Organometallic Compounds / pharmacology*
  • Phenanthrolines / chemistry*
  • Prion Proteins
  • Prions / metabolism
  • Thiones / chemistry*
  • X-Ray Diffraction

Substances

  • Cations, Divalent
  • Imidazolidines
  • Organometallic Compounds
  • Phenanthrolines
  • Prion Proteins
  • Prions
  • Prnp protein, mouse
  • Thiones
  • Ethylenethiourea
  • Copper