Abstract
Map based cloning in Arabidopsis thaliana can be a difficult and time-consuming process, specifically if the phenotype is subtle and scoring labour intensive. Here, we have re-sequenced the 120-Mb genome of a novel Arabidopsis clock mutant early bird (ebi-1) in Wassilewskija (Ws-2). We demonstrate the utility of sequencing a backcrossed line in limiting the number of SNPs considered. We identify a SNP in the gene AtNFXL-2 as the likely cause of the ebi-1 phenotype.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arabidopsis / genetics*
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Arabidopsis / metabolism
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Chromosome Mapping
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Circadian Clocks / genetics*
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Conserved Sequence
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Ethyl Methanesulfonate / pharmacology
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Gene Expression Regulation, Plant
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Genome, Plant*
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Molecular Sequence Data
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Mutagens / pharmacology
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Mutation / genetics*
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Phenotype
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Polymorphism, Single Nucleotide / drug effects
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Reproducibility of Results
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Sequence Alignment
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Sequence Analysis, DNA*
Substances
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Arabidopsis Proteins
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Mutagens
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Ethyl Methanesulfonate