Detection of seasonal H3N2 influenza A virus by type-specific TaqMan minor groove binder probe assay

Diagn Microbiol Infect Dis. 2011 Jun;70(2):281-4. doi: 10.1016/j.diagmicrobio.2011.01.012. Epub 2011 Mar 22.

Abstract

Despite the emergence of the pandemic H1N1 influenza A virus in 2009, seasonal H3N2 viruses continue to co-circulate in the population and may even predominate in the coming influenza season. We describe a specific minor groove binder TaqMan assay for H3N2 viruses with a detection limit of 16.5 standard DNA copies.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Intramural

MeSH terms

  • Humans
  • Influenza A Virus, H3N2 Subtype / genetics
  • Influenza A Virus, H3N2 Subtype / isolation & purification*
  • Influenza, Human / diagnosis*
  • Influenza, Human / virology*
  • Molecular Diagnostic Techniques / methods*
  • Oligonucleotide Probes / genetics*
  • RNA, Viral / genetics*
  • Sensitivity and Specificity
  • Virology / methods*

Substances

  • Oligonucleotide Probes
  • RNA, Viral