Neriifolin from seeds of Cerbera manghas L. induces cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells

Fitoterapia. 2011 Jul;82(5):735-41. doi: 10.1016/j.fitote.2011.03.004. Epub 2011 Mar 31.

Abstract

The effects of neriifolin, a cardiac glycoside from Cerbera manghas L. on various end-points of oncologic relevance (cell growth, cell cycle regulation, and apoptosis in HepG2 cells) were investigated. Neriifolin reduced viability of HepG2 cells, induced S and G2/M phase arrests of the cell cycle, and stimulated apoptosis of HepG2 cells. Stimulation of HepG2 cells with neriifolin induced activation of caspase-3, -8, and -9, and up-regulated expression of Fas and FasL proteins. Based on these results, neriifolin could be considered a candidate for the treatment of hepatocellular carcinoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Antineoplastic Agents, Phytogenic / therapeutic use
  • Apocynaceae / chemistry*
  • Apoptosis / drug effects*
  • Carcinoma, Hepatocellular / drug therapy*
  • Carcinoma, Hepatocellular / metabolism
  • Cardenolides / isolation & purification
  • Cardenolides / pharmacology
  • Cardenolides / therapeutic use*
  • Caspases / metabolism
  • Cell Cycle / drug effects*
  • Fas Ligand Protein / metabolism
  • Fas-Associated Death Domain Protein / metabolism
  • Hep G2 Cells
  • Humans
  • Liver Neoplasms / drug therapy*
  • Liver Neoplasms / metabolism
  • Phytotherapy*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Seeds
  • Up-Regulation

Substances

  • Antineoplastic Agents, Phytogenic
  • Cardenolides
  • Fas Ligand Protein
  • Fas-Associated Death Domain Protein
  • Plant Extracts
  • neriifolin
  • Caspases