Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver

Biochem Biophys Res Commun. 2011 Apr 29;408(1):120-5. doi: 10.1016/j.bbrc.2011.03.133. Epub 2011 Apr 2.

Abstract

After partial hepatectomy (PH), regenerating liver accumulates unknown lipid species. Here, we analyzed lipids in murine liver and adipose tissues following PH by thin-layer chromatography (TLC), imaging mass spectrometry (IMS), and real-time RT-PCR. In liver, IMS revealed that a single TLC band comprised major 19 TG species. Similarly, IMS showed a single phospholipid TLC band to be major 13 species. In adipose tissues, PH induced changes to expression of genes regulating lipid metabolism. Finally, IMS of phosphatidylcholine species demonstrated distribution gradients in lobules that resembled hepatic zonation. IMS is thus a novel and power tool for analyzing lipid species with high resolution.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Thin Layer
  • Female
  • Liver / chemistry*
  • Liver / metabolism
  • Liver Regeneration*
  • Mice
  • Mice, Inbred C57BL
  • Phospholipids / analysis*
  • Phospholipids / metabolism
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Triglycerides / analysis*
  • Triglycerides / metabolism

Substances

  • Phospholipids
  • Triglycerides