Forced needle advancement during needle-nerve contact in a porcine model: histological outcome

Anesth Analg. 2011 Aug;113(2):417-20. doi: 10.1213/ANE.0b013e31821b2227. Epub 2011 Apr 5.

Abstract

Background: In this study, we determined whether needle advancement during needle-nerve contact (forced needle-nerve contact) is associated with a higher risk of nerve injury compared with needle-nerve contact without needle advancement (nonforced needle-nerve contact).

Methods: In 8 anesthetized pigs, the brachial plexus nerves underwent forced (0.15 Newton) or nonforced (0.0 Newton) needle-nerve contact without nerve penetration. The grade of nerve injury was histologically assessed using an objective score ranging from 0 (no injury) to 4 (severe injury).

Results: Sixty-nine nerves, including controls, were examined. Histology revealed a significant difference between forced and nonforced needle-nerve contact (median [interquartile range] 3 [2-4] vs 2 [1-2]; P = 0.004). Myelin damage and intraneural hematoma occurred only after forced needle-nerve contact.

Conclusions: The severity of structural nerve injury after needle-nerve contact was directly related to force exposure via needle advancement.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anesthesia, Conduction / instrumentation*
  • Animals
  • Brachial Plexus / anatomy & histology
  • Hematoma / pathology
  • Immunohistochemistry
  • Inflammation / pathology
  • Myelin Sheath / pathology
  • Needles*
  • Peripheral Nerve Injuries
  • Peripheral Nerves / anatomy & histology
  • Peripheral Nerves / pathology
  • Swine