Role of interleukin 17 in inflammation, atherosclerosis, and vascular function in apolipoprotein e-deficient mice

Arterioscler Thromb Vasc Biol. 2011 Jul;31(7):1565-72. doi: 10.1161/ATVBAHA.111.227629. Epub 2011 Apr 7.

Abstract

Objective: Interleukin 17A (IL17A) is involved in many inflammatory processes, but its role in atherosclerosis remains controversial. We examined the role of IL17A in mouse and human atherosclerosis.

Methods and results: Atherosclerosis was induced in apolipoprotein E (ApoE)(-/-) and IL17A/ApoE(-/-) mice using high-fat feeding, angiotensin II infusion, or partial carotid ligation. In ApoE(-/-) mice, 3 months of high-fat diet induced interferon-γ production by splenic lymphocytes, and this was abrogated in IL17A/ApoE(-/-) mice. IL17A/ApoE(-/-) mice had reduced aortic superoxide production, increased aortic nitric oxide levels, decreased aortic leukocyte and dendritic cell infiltration, and reduced weight gain after a high-fat diet compared with ApoE(-/-) mice. Despite these favorable effects, IL17A deficiency did not affect aortic plaque burden after a high-fat diet or angiotensin II infusion. In a partial carotid ligation model, IL17A deficiency did not affect percentage of stenosis but reduced outward remodeling. In this model, neutralization of the related isoform, IL17F, in IL17A/ApoE(-/-) mice did not alter atherosclerosis. Finally, there was no correlation between IL17A levels and carotid intima-media thickness in humans.

Conclusions: IL17 contributes to vascular and systemic inflammation in experimental atherosclerosis but does not alter plaque burden. The changes in plaque composition caused by IL17 might modulate plaque stability.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aged
  • Angiotensin II
  • Animals
  • Antibodies, Neutralizing / administration & dosage
  • Aorta / immunology
  • Aorta / metabolism
  • Aortic Diseases / genetics
  • Aortic Diseases / immunology*
  • Aortic Diseases / metabolism
  • Aortic Diseases / pathology
  • Apolipoproteins E / deficiency*
  • Apolipoproteins E / genetics
  • Atherosclerosis / genetics
  • Atherosclerosis / immunology*
  • Atherosclerosis / metabolism
  • Atherosclerosis / pathology
  • Carotid Arteries / surgery
  • Carotid Artery Diseases / genetics
  • Carotid Artery Diseases / immunology*
  • Carotid Artery Diseases / metabolism
  • Carotid Artery Diseases / pathology
  • Cells, Cultured
  • Dendritic Cells / immunology
  • Dietary Fats
  • Disease Models, Animal
  • Female
  • Humans
  • Inflammation / genetics
  • Inflammation / immunology*
  • Inflammation / metabolism
  • Inflammation / pathology
  • Interferon-gamma / metabolism
  • Interleukin-17 / blood*
  • Interleukin-17 / deficiency
  • Interleukin-17 / genetics
  • Interleukin-17 / immunology
  • Interleukin-17 / metabolism*
  • Ligation
  • Lymphocytes / immunology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Middle Aged
  • Nitric Oxide / metabolism
  • Spleen / immunology
  • Superoxides / metabolism
  • Time Factors

Substances

  • Antibodies, Neutralizing
  • Apolipoproteins E
  • Dietary Fats
  • IL17A protein, human
  • Il17a protein, mouse
  • Il17f protein, mouse
  • Interleukin-17
  • Superoxides
  • Angiotensin II
  • Nitric Oxide
  • Interferon-gamma