Industrial production of fructooligosaccharides by immobilized cells of Aureobasidium pullulans in a packed bed reactor

K H Jung; S H Bang; T K Oh; H J Park

doi:10.1007/s10529-011-0606-8

Industrial production of fructooligosaccharides by immobilized cells of Aureobasidium pullulans in a packed bed reactor

Biotechnol Lett. 2011 Aug;33(8):1621-4. doi: 10.1007/s10529-011-0606-8. Epub 2011 Apr 9.

Authors

K H Jung¹, S H Bang, T K Oh, H J Park

Affiliation

¹ Technical Center for Bio-Business Unit, CJ Cheiljedang Corporation, 92 Kayang-Dong, Kangseo-Ku, Seoul, Korea.

PMID: 21479630
DOI: 10.1007/s10529-011-0606-8

Abstract

Continuous production of fructooligosaccharides (FOS) by Aureobasidium pullulans immobilized on calcium alginate beads with a packed bed was investigated at a plant scale reactor. Optimum conditions were with 770 g sucrose/l, being fed at 200 l/h at 50°C which gave a productivity of 180 g FOS/l h. Initial activity was maintained for more than 100 days. The reactor was successfully scaled up to a production scale of 1.2 m(3).

MeSH terms

Ascomycota / enzymology
Ascomycota / metabolism*
Bioreactors / microbiology*
Cells, Immobilized / enzymology
Cells, Immobilized / metabolism*
Enzyme Stability
Glucose / analysis
Glucose / metabolism
Hexosyltransferases / metabolism
Industrial Microbiology / instrumentation*
Industrial Microbiology / methods*
Oligosaccharides / analysis
Oligosaccharides / metabolism*
Sucrose / metabolism
Trisaccharides / metabolism

Substances

Oligosaccharides
Trisaccharides
1-kestose
fungitetraose
Sucrose
Hexosyltransferases
inulosucrase
Glucose