Differential levels of resistance to disease induction and development of relapsing experimental autoimmune encephalomyelitis in two H-2b-restricted mouse strains

J Neuroimmunol. 2011 May;234(1-2):109-14. doi: 10.1016/j.jneuroim.2011.03.008. Epub 2011 Apr 11.

Abstract

Besides the major histocompatibility complex (MHC) genes, background genes are believed to influence the encephalitogenicity of SJL(H-2(s)) and B10.S (H-2(s)) mice responding to myelin basic protein (MBP). A new mouse strain was constructed to study the effects of the SJL genetic background in mice responding to H-2(b)-restricted neuroantigens. Although the SJL.B (H-2(b)) mouse remained resistant to MBP in active EAE induction, the disease severity was uniformly higher in MOG-induced active EAE and in MBP-induced adoptive EAE when compared to those of B6 (H-2(b)) mice. Treatment of mice with anti-CD25 antibodies prior to immunization caused 60% of SJL.B mice to become susceptible to MBP-induced EAE while only 14% of B6 mice were converted. In addition, MOG-induced EAE in SJL.B mice followed a remitting-relapsing disease course while B6 mice only exhibited monophasic or chronic episodes. The new SJL.B mouse strain provides a valuable tool for studying EAE resistance and remitting-relapsing disease in H-2(b) mice.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antibodies / therapeutic use
  • Cytokines / metabolism
  • Disease Models, Animal
  • Disease Susceptibility / immunology*
  • Encephalomyelitis, Autoimmune, Experimental / drug therapy
  • Encephalomyelitis, Autoimmune, Experimental / etiology
  • Encephalomyelitis, Autoimmune, Experimental / genetics*
  • Encephalomyelitis, Autoimmune, Experimental / immunology*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Female
  • H-2 Antigens / genetics*
  • Interleukin-2 Receptor alpha Subunit / immunology
  • Mice
  • Mice, Inbred C57BL
  • Myelin Basic Protein / immunology
  • Myelin Basic Protein / toxicity
  • Peptide Fragments / immunology
  • Peptide Fragments / toxicity
  • Species Specificity
  • Time Factors

Substances

  • Antibodies
  • Cytokines
  • H-2 Antigens
  • Interleukin-2 Receptor alpha Subunit
  • Myelin Basic Protein
  • Peptide Fragments