A quantitative literature-curated gold standard for kinase-substrate pairs

Genome Biol. 2011;12(4):R39. doi: 10.1186/gb-2011-12-4-r39. Epub 2011 Apr 14.

Abstract

We describe the Yeast Kinase Interaction Database (KID, http://www.moseslab.csb.utoronto.ca/KID/), which contains high- and low-throughput data relevant to phosphorylation events. KID includes 6,225 low-throughput and 21,990 high-throughput interactions, from greater than 35,000 experiments. By quantitatively integrating these data, we identified 517 high-confidence kinase-substrate pairs that we consider a gold standard. We show that this gold standard can be used to assess published high-throughput datasets, suggesting that it will enable similar rigorous assessments in the future.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Databases, Protein*
  • Protein Kinases / classification
  • Protein Kinases / metabolism*
  • Reference Standards
  • Saccharomyces cerevisiae / enzymology*
  • Substrate Specificity

Substances

  • Protein Kinases