Virus-induced differential expression of nuclear receptors and coregulators in dendritic cells: implication to interferon production

FEBS Lett. 2011 May 6;585(9):1331-7. doi: 10.1016/j.febslet.2011.04.001. Epub 2011 Apr 7.

Abstract

We investigated mRNA expression of 49 nuclear hormone receptors (NRs) and 35 transcriptional coregulators in mouse bone marrow-derived dendritic cells (DCs) upon infection with Newcastle Disease virus (NDV) or murine cytomegalovirus (MCMV). These viruses regulated mRNA expression of some NRs among which NOR1 and LXRα were highly induced at mRNA and protein levels. Exogenous expression of the latter NRs repressed IRF3- or IRF7-induced transactivation of the interferon β promoter and NDV infection further potentiated their repressive effect. The viral infection also significantly regulated mRNA expression of some coregulators, including HDAC1. Toll-like receptor ligands regulated NR and coregulator mRNA expression similar to the viruses. Thus, NRs and coregulators are integral components of DC-organizing anti-viral response wherein NOR1 and LXRα participate in regulating interferon production.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Cells, Cultured
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Dendritic Cells / metabolism*
  • Dendritic Cells / virology*
  • Gene Expression Profiling
  • HCT116 Cells
  • Histone Deacetylase 1 / genetics
  • Histone Deacetylase 1 / metabolism*
  • Host-Pathogen Interactions
  • Humans
  • Immunoblotting
  • Interferon-alpha / genetics
  • Interferon-alpha / metabolism
  • Liver X Receptors
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Muromegalovirus / physiology*
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Newcastle disease virus / physiology*
  • Orphan Nuclear Receptors / genetics
  • Orphan Nuclear Receptors / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Cytoplasmic and Nuclear / genetics
  • Receptors, Cytoplasmic and Nuclear / metabolism*
  • Receptors, Steroid / genetics
  • Receptors, Steroid / metabolism
  • Receptors, Thyroid Hormone / genetics
  • Receptors, Thyroid Hormone / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • DNA-Binding Proteins
  • Interferon-alpha
  • Liver X Receptors
  • NR1H3 protein, human
  • Nerve Tissue Proteins
  • Nr1h3 protein, mouse
  • Nr4a3 protein, mouse
  • Orphan Nuclear Receptors
  • RNA, Messenger
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Steroid
  • Receptors, Thyroid Hormone
  • Hdac1 protein, mouse
  • Histone Deacetylase 1