Automated radiosynthesis of the thiol-reactive labeling agent N-[6-(4-[18F]fluorobenzylidene)aminooxyhexyl]maleimide ([18F]FBAM)

Torsten Kniess; Manuela Kuchar; Jens Pietzsch

doi:10.1016/j.apradiso.2011.03.043

Automated radiosynthesis of the thiol-reactive labeling agent N-[6-(4-[18F]fluorobenzylidene)aminooxyhexyl]maleimide ([18F]FBAM)

Appl Radiat Isot. 2011 Sep;69(9):1226-30. doi: 10.1016/j.apradiso.2011.03.043. Epub 2011 Apr 8.

Authors

Torsten Kniess¹, Manuela Kuchar, Jens Pietzsch

Affiliation

¹ Institute of Radiopharmacy, Forschungszentrum Dresden-Rossendorf e.V., Germany. [email protected]

PMID: 21507666
DOI: 10.1016/j.apradiso.2011.03.043

Abstract

The two-step radiosynthesis of N-[6-(4-[(18)F]fluorobenzylidene)aminooxyhexyl]maleimide ([(18)F]FBAM) was adapted to a remotely controlled synthesizer module. After optimization of reaction conditions as well as solid phase extraction based purification steps, the final [(18)F]FBAM was obtained in a decay-corrected radiochemical yield of 29±4% (related to [(18)F]fluoride, n=12) within a total synthesis time of 40 min. The radiochemical purity of [(18)F]FBAM was in the range 94-98%, the specific activity was determined with 13.4-17.2 GBq/μmol.

MeSH terms

Automation, Laboratory / methods
Fluorine Radioisotopes*
Isotope Labeling / methods*
Maleimides / chemical synthesis*
Radiopharmaceuticals / chemical synthesis*
Sulfhydryl Reagents / chemical synthesis*

Substances

Fluorine Radioisotopes
Maleimides
N-(6-(4-fluorobenzylidene)aminooxyhexyl)maleimide
Radiopharmaceuticals
Sulfhydryl Reagents