Abstract
Eggs of the common barbel (Barbus barbus) cause intoxication in humans after ingestion. In this work, the chemical composition of the haemolytically active fraction from methanolic barbel roe extract was analyzed. Compounds showing haemolytic activity and cytotoxicity towards normal and transformed cell lines were isolated and identified as polyunsaturated fatty acids, using online liquid chromatography-electrospray ionization mass spectrometry through tandem fragmentation experiments (HPLC-MS/MS). Arachidonic acid (C20:4), docosahexaenoic acid (C22:6) and eicosapentaenoic acid (C20:5) proved to be the three most abundant members of a complex series of free fatty acids ranging from C14:0 to C24:5.
Copyright © 2011 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arachidonic Acid / analysis
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Arachidonic Acid / chemistry
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Arachidonic Acid / metabolism
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Cell Line, Transformed
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Chromatography, High Pressure Liquid
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Cyprinidae / metabolism*
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Docosahexaenoic Acids / analysis
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Docosahexaenoic Acids / chemistry
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Docosahexaenoic Acids / metabolism
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Eicosapentaenoic Acid / analysis
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Eicosapentaenoic Acid / chemistry
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Eicosapentaenoic Acid / metabolism
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Evaluation Studies as Topic
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Fatty Acids, Unsaturated / analysis*
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Fatty Acids, Unsaturated / chemistry
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Fatty Acids, Unsaturated / metabolism
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Female
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Hemolysis*
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Humans
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Methanol / chemistry
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Ovum / chemistry
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Ovum / metabolism*
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Spectrometry, Mass, Electrospray Ionization
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Toxicity Tests
Substances
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Fatty Acids, Unsaturated
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Docosahexaenoic Acids
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Arachidonic Acid
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Eicosapentaenoic Acid
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Methanol