Screening and identification of host factors interacting with UL14 of herpes simplex virus 1

Med Microbiol Immunol. 2011 Aug;200(3):203-8. doi: 10.1007/s00430-011-0196-z. Epub 2011 Apr 22.

Abstract

The UL14 protein of herpes simplex virus type 1 (HSV-1) is highly conserved in herpesvirus family. However, its exact function during the HSV-1 replication cycle is little known. In the present study, a high throughput yeast two-hybrid system was employed to screen the cellular factors interacting with UL14, and five target candidates were yielded: (1) TSC22 domain family protein 3 (TSC22D3); (2) Mediator of RNA polymerase II transcription subunit 8 isoform 1(MED8); (3) Runt-related transcription factor 3 (RUNX3); (4) Arrestin beta-2 (ARRB2); (5) Cereblon (CRBN). Indirect immunofluorescent assay showed that both TSC22D3 and MED8 co-localized with UL14. Co-immunoprecipitation assay demonstrated that UL14 could be immunoprecipitated by TSC22D3, suggesting that UL14 interacted with TSC22D3 under physiological condition. In summary, this study opened up new avenues toward delineating the function and physiological significance of UL14 during the HSV-1 replication cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Arrestins / genetics
  • Arrestins / metabolism
  • COS Cells
  • Chlorocebus aethiops
  • Core Binding Factor Alpha 3 Subunit / genetics
  • Core Binding Factor Alpha 3 Subunit / metabolism
  • Fluorescent Antibody Technique, Indirect
  • HEK293 Cells
  • Herpesvirus 1, Human / genetics
  • Herpesvirus 1, Human / metabolism
  • Herpesvirus 1, Human / physiology
  • Host-Pathogen Interactions*
  • Humans
  • Immunoprecipitation
  • Mediator Complex / genetics
  • Mediator Complex / metabolism*
  • Peptide Hydrolases / genetics
  • Peptide Hydrolases / metabolism
  • Plasmids / genetics
  • Plasmids / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Two-Hybrid System Techniques
  • Ubiquitin-Protein Ligases
  • Vero Cells
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*
  • Virus Replication
  • beta-Arrestin 2
  • beta-Arrestins

Substances

  • ARRB2 protein, human
  • Adaptor Proteins, Signal Transducing
  • Arrestins
  • CRBN protein, human
  • Core Binding Factor Alpha 3 Subunit
  • MED8 protein, human
  • Mediator Complex
  • Runx3 protein, human
  • TSC22D3 protein, human
  • Transcription Factors
  • UL14 protein, Human herpesvirus 1
  • Viral Proteins
  • beta-Arrestin 2
  • beta-Arrestins
  • Ubiquitin-Protein Ligases
  • Peptide Hydrolases