Use of nested polymerase chain reaction based on sequence-based typing of clinical samples to determine the source of infection for hospital-acquired Legionnaires' disease

Infect Control Hosp Epidemiol. 2011 May;32(5):510-2. doi: 10.1086/659785.

Abstract

The source of infection of a hospital-acquired Legionnaires' disease case was determined for the first time by nested polymerase chain reaction based on sequence-based typing. The typing was performed directly on DNA extracted from tissue samples, allowing a rapid epidemiological correlation with environmental isolates.

Publication types

  • Case Reports

MeSH terms

  • Cross Infection / microbiology*
  • DNA, Bacterial / analysis
  • Fatal Outcome
  • Female
  • Humans
  • Legionella pneumophila / genetics*
  • Legionella pneumophila / isolation & purification
  • Legionnaires' Disease / microbiology*
  • Legionnaires' Disease / transmission
  • Middle Aged
  • Polymerase Chain Reaction / methods*

Substances

  • DNA, Bacterial