Molecular characterization of drug-resistant Mycobacterium tuberculosis isolates circulating in China by multilocus PCR and electrospray ionization mass spectrometry

J Clin Microbiol. 2011 Jul;49(7):2719-21. doi: 10.1128/JCM.00317-11. Epub 2011 Apr 27.

Abstract

We used multilocus PCR and electrospray ionization mass spectrometry (PCR/ESI-MS) to determine the genotype and drug resistance profiles for 96 Mycobacterium tuberculosis isolates circulating in regions of high and low tuberculosis (TB) endemicity in China. The dominant principal genetic group (PGG) circulating in China was PGG1, and drug-resistant gene mutations were more diversified in the region of low rather than high TB endemicity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antitubercular Agents / pharmacology
  • Bacterial Proteins / genetics
  • China / epidemiology
  • DNA, Bacterial / genetics
  • Drug Resistance, Bacterial
  • Endemic Diseases
  • Genotype
  • Humans
  • Molecular Typing / methods*
  • Mutation, Missense
  • Mycobacterium tuberculosis / chemistry*
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / isolation & purification
  • Polymerase Chain Reaction / methods*
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Tuberculosis, Multidrug-Resistant / epidemiology*
  • Tuberculosis, Multidrug-Resistant / microbiology*

Substances

  • Antitubercular Agents
  • Bacterial Proteins
  • DNA, Bacterial