cTAGE5 mediates collagen secretion through interaction with TANGO1 at endoplasmic reticulum exit sites

Mol Biol Cell. 2011 Jul 1;22(13):2301-8. doi: 10.1091/mbc.E11-02-0143. Epub 2011 Apr 27.

Abstract

Cutaneous T-cell lymphoma-associated antigen 5 (cTAGE5), an originally identified tumor antigen, is overexpressed in various cancer cell lines. The cDNA encodes an integral membrane protein containing two coiled-coil motifs and a proline-rich domain. We show that cTAGE5 specifically localizes to the endoplasmic reticulum (ER) exit sites. In addition, cTAGE5 forms a complex with TANGO1 (MIA3), a previously characterized cargo receptor for collagen VII, by the interaction of their coiled-coil motifs. Of interest, cTAGE5, as well as TANGO1, is capable of interacting with the inner-layer coatomer of COPII Sec23/24 complex through their C-terminal proline-rich domains and required for collagen VII secretion. We propose that cTAGE5 acts as a coreceptor of TANGO1 for collagen VII export from the ER.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Neoplasm / metabolism*
  • Aryl Hydrocarbon Receptor Nuclear Translocator / metabolism*
  • COP-Coated Vesicles / metabolism
  • Carrier Proteins / metabolism
  • Cell Line, Transformed
  • Collagen / metabolism*
  • Endoplasmic Reticulum / metabolism*
  • HeLa Cells
  • Humans
  • Membrane Proteins / metabolism
  • Neoplasm Proteins / metabolism*
  • Protein Binding
  • Protein Transport
  • Receptors, Cell Surface / metabolism

Substances

  • ARNT protein, human
  • Antigens, Neoplasm
  • Carrier Proteins
  • MIA2 protein, human
  • Membrane Proteins
  • Neoplasm Proteins
  • Receptors, Cell Surface
  • serpin-enzyme complex receptor
  • Aryl Hydrocarbon Receptor Nuclear Translocator
  • Collagen