Abstract
In this study, the sperm protein profile was compared between fertile and infertile men using 2-dimensional gel electrophoresis, liquid chromatography mass spectrometer analysis, and matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry. Four unique proteins, semenogelin II precursor, prolactin-induced protein, clusterin isoform 1, and prostate-specific antigen isoform 1 preproprotein, were predominantly present in the semen of healthy men; however, semenogelin II precursor and clusterin isoform 1 were not seen in the semen of infertile men, suggesting unique differences in the spermatozoa protein profiles of fertile and infertile men.
Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.
MeSH terms
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Amino Acid Sequence
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Biomarkers / analysis
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Carrier Proteins / analysis
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Case-Control Studies
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Chromatography, Reverse-Phase
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Clusterin / analysis
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Electrophoresis, Gel, Two-Dimensional
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Fertility*
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Glycoproteins / analysis
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Humans
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Infertility, Male / metabolism*
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Infertility, Male / physiopathology
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Male
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Membrane Transport Proteins
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Molecular Weight
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Ohio
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Pilot Projects
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Prostate-Specific Antigen / analysis
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Proteomics* / methods
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Seminal Plasma Proteins / analysis*
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Seminal Plasma Proteins / chemistry
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Seminal Vesicle Secretory Proteins / analysis
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Spermatozoa / chemistry*
Substances
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Biomarkers
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CLU protein, human
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Carrier Proteins
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Clusterin
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Glycoproteins
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Membrane Transport Proteins
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PIP protein, human
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Seminal Plasma Proteins
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Seminal Vesicle Secretory Proteins
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seminal vesicle-specific antigen
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Prostate-Specific Antigen