Interferon-γ release assay for the diagnosis of tuberculosis in children

Arch Dis Child. 2012 Jun;97(6):514-6. doi: 10.1136/adc.2010.202069. Epub 2011 May 4.

Abstract

Aims: To compare the QuantiFERON-TB GOLD In Tube test (QTF) and the tuberculin skin test (TST) in children.

Methods: A prospective study was carried out in nine hospitals in Madrid, Spain. TST and QTF were performed in immigrants, tuberculosis (TB) contacts and patients with TB disease (TBD).

Results: 459 children were included. Disagreement between the tests was more frequently observed among latent tuberculosis infection (LTBI) cases (54%; 38/70) than in non-infected or TBD cases (0.8%; 3/369) (p<0.01). There were more BCG-vaccinated children among LTBI cases with negative QTF (76%) than among LTBI cases with positive QTF (40%) (p<0.001). Agreement between tests in BCG-vaccinated children was lower than in non-vaccinated cases (p<0.05). Tests in TB exposed patients showed better agreement than in non-exposed children (p<0.05).

Conclusions: Agreement of both tests was excellent in TBD cases, non-vaccinated children and non-infected patients. A significant number of QTF negative results were observed among LTBI cases, especially in BCG-vaccinated children. Agreement was better in exposed children.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child
  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay* / methods
  • Humans
  • Interferon-gamma Release Tests* / methods
  • Mass Screening / methods*
  • Multivariate Analysis
  • Mycobacterium tuberculosis
  • Prospective Studies
  • Tuberculin Test* / methods
  • Tuberculosis / diagnosis*
  • Tuberculosis / prevention & control