Abstract
Enzyme-responsive, hybrid, magnetic silica nanoparticles have been employed for multifunctional applications in selective drug delivery and intracellular tumor imaging. In this study, doxorubicin (Dox)-conjugated, enzyme-cleavable peptide precursors were covalently tethered onto the surface of uniform silica-coated magnetic nanoparticles through click chemistry. This enzyme-responsive nanoparticle conjugate demonstrated highly efficient Dox release upon specific enzyme interactions in vitro. It also exhibits multiple functions in selective tumor intracellular drug delivery and imaging in the tumor cells with high cathepsin B expression, whereas it exhibited lower cytotoxicity towards other cells without enzyme expression.
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibiotics, Antineoplastic / administration & dosage
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Antibiotics, Antineoplastic / chemistry*
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Antibiotics, Antineoplastic / toxicity
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Cathepsin B / metabolism*
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Cell Line, Tumor
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Click Chemistry
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Doxorubicin / administration & dosage
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Doxorubicin / chemistry*
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Doxorubicin / toxicity
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Drug Carriers / chemical synthesis
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Drug Carriers / chemistry*
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Ferric Compounds / chemistry
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Humans
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Magnetic Resonance Imaging
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Magnetite Nanoparticles / chemistry*
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Mice
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Microscopy, Fluorescence
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Neoplasms / diagnosis
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Neoplasms / drug therapy
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Neoplasms / enzymology
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Peptides / chemistry
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Silicon Dioxide / chemistry
Substances
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Antibiotics, Antineoplastic
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Drug Carriers
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Ferric Compounds
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Magnetite Nanoparticles
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Peptides
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ferric oxide
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Silicon Dioxide
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Doxorubicin
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Cathepsin B