Objective: A molecular biology method was studied to identify medical Dendrobium and to provide a method for quality control of these plants.
Method: ISSR primer was screened through ISSR-PCR reaction according to its gene resolving power, and digital barcodes were established for identification.
Results: Screening 2 primers which Rp value above 8, This primers can identify medical Dendrobium from 6 kinds 8 groups.
Conclusion: ISSR molecular maker technology is useful for identifying species and habitats of medical Dendrobium plants.