The ammonium sulfate activation of phosphorylase b

FEBS Lett. 1990 Feb 12;261(1):23-7. doi: 10.1016/0014-5793(90)80627-u.

Abstract

The ammonium sulfate activation of phosphorylase b has been studied. Ammonium sulfate, when present in high concentrations, induces properties of phosphorylase a in phosphorylase b, such as an enhanced affinity for AMP, a reversal of the glucose-6-P inhibition and enzyme tetramerization. The data are consistent with the interpretation that sulfates bind to the Ser-14 site and the sulfate-protein interactions at this site are responsible for activation of phosphorylase b.

MeSH terms

  • Adenosine Monophosphate / pharmacology
  • Ammonium Sulfate / pharmacology*
  • Animals
  • Electron Spin Resonance Spectroscopy
  • Enzyme Activation / drug effects
  • Glucosephosphates / metabolism
  • Kinetics
  • Macromolecular Substances
  • Muscles / enzymology*
  • Phosphorylase b / metabolism*
  • Phosphorylases / metabolism*
  • Rabbits
  • Ultracentrifugation

Substances

  • Glucosephosphates
  • Macromolecular Substances
  • Adenosine Monophosphate
  • glucose-1-phosphate
  • Phosphorylase b
  • Phosphorylases
  • Ammonium Sulfate