Quantitative assessment of changes in trabeculectomy blebs after laser suture lysis using anterior segment coherence tomography

J Glaucoma. 2012 Jun-Jul;21(5):313-7. doi: 10.1097/IJG.0b013e31820e2d23.

Abstract

Purpose: To quantify changes in bleb morphology after laser suture lysis (LSL) using anterior segment optical coherence tomography (AS-OCT).

Patients and methods: This was a prospective observational case series of patients who underwent trabeculectomy surgery with mitomycin-C and required subsequent LSL. Subjects were assessed before and immediately after LSL, and at 3 months after LSL. At each visit, a standardized cross-sectional AS-OCT image of the bleb was obtained, from which a masked observer measured bleb height (hB), cavity height (hC), wall cross sectional area (AW), cavity cross sectional area (AC), and bleb cross sectional area (AB) using custom software.

Results: Twenty-five subjects (25 eyes) were examined. LSL was performed at a mean of 21.5±16.3 days after trabeculectomy. Immediately after LSL, there was a significant increase in mean hB (P=0.026) and mean hC (P=0.45) from pre-LSL. At 3 months after LSL, there was a significant increase in mean hB (P=0.003), mean AC (P=0.047), and mean AB (P=0.006) as compared with before LSL. Increase in hB and hC immediately after LSL was predictive of outcome at month 3.

Conclusions: AS-OCT was able to quantify changes in bleb morphology after LSL, some of which were predictive of eventual outcome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkylating Agents / administration & dosage
  • Aqueous Humor / metabolism
  • Conjunctiva / metabolism
  • Conjunctiva / pathology*
  • Female
  • Glaucoma / metabolism
  • Glaucoma / surgery*
  • Humans
  • Intraocular Pressure
  • Laser Therapy / methods*
  • Lasers, Solid-State / therapeutic use
  • Male
  • Middle Aged
  • Mitomycin / administration & dosage
  • Phacoemulsification
  • Prospective Studies
  • Sclera / surgery
  • Surgical Flaps / pathology*
  • Suture Techniques*
  • Tomography, Optical Coherence*
  • Trabeculectomy*

Substances

  • Alkylating Agents
  • Mitomycin