A universal code for RNA recognition by PUF proteins

Aleksandra Filipovska; Muhammad F M Razif; Karoline K A Nygård; Oliver Rackham

doi:10.1038/nchembio.577

A universal code for RNA recognition by PUF proteins

Nat Chem Biol. 2011 May 15;7(7):425-7. doi: 10.1038/nchembio.577.

Authors

Aleksandra Filipovska¹, Muhammad F M Razif, Karoline K A Nygård, Oliver Rackham

Affiliation

¹ Western Australian Institute for Medical Research and Centre for Medical Research, The University of Western Australia, Perth, Australia.

PMID: 21572425
DOI: 10.1038/nchembio.577

Abstract

The design of proteins that can bind any RNA sequence of interest has many potential biological and medical applications. Here we have expanded the recognition of Pumilio and FBF homology protein (PUF) repeats beyond adenine, guanine and uracil and evolved them to specifically bind cytosine. These repeat sequences can be used to create PUF domains capable of selectively binding RNA targets of diverse sequence and structure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cytosine / metabolism*
Electrophoretic Mobility Shift Assay
Humans
Mutation
Protein Engineering*
RNA / chemistry
RNA / genetics
RNA / metabolism*
RNA-Binding Proteins / chemistry
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism*
Repetitive Sequences, Amino Acid
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Two-Hybrid System Techniques

Substances

PUM1 protein, human
RNA-Binding Proteins
RNA
Cytosine