Abstract
A Fischer carbene complex was grafted onto self-assembled monolayers (SAMs) on gold or glass by a copper-free "click" reaction. Pendant lysine residues of protein A obtained from Staphylococcus aureus rapidly reacted with the electrophilic metal complex on SAM effecting a covalent attachment of protein A with the surface. The protein A coated surface further led to bioaffinity immobilization of rabbit IgG in an oriented manner, a feature that also permits its purification from rabbit serum. Rabbit IgG could be removed from protein A coated surface by pH adjustment. The regenerated protein A surface was reused three times without loss of activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Click Chemistry
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Glass / chemistry
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Gold / chemistry
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Hydrogen-Ion Concentration
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Immunoglobulin G / blood
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Immunoglobulin G / immunology*
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Immunoglobulin G / isolation & purification
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Membranes, Artificial*
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Methane / analogs & derivatives*
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Methane / chemistry
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Molecular Structure
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Organometallic Compounds / chemical synthesis
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Organometallic Compounds / chemistry
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Organometallic Compounds / immunology*
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Particle Size
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Rabbits
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Staphylococcal Protein A / chemistry
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Staphylococcal Protein A / immunology*
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Staphylococcus aureus / chemistry
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Stereoisomerism
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Surface Properties
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Tungsten / chemistry
Substances
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Immunoglobulin G
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Membranes, Artificial
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Organometallic Compounds
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Staphylococcal Protein A
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carbene
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Gold
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Methane
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Tungsten