Selective labeling and monitoring pH changes of lysosomes in living cells with fluorogenic pH sensors

Lai-Qiang Ying; Bruce P Branchaud

doi:10.1016/j.bmcl.2011.04.137

Selective labeling and monitoring pH changes of lysosomes in living cells with fluorogenic pH sensors

Bioorg Med Chem Lett. 2011 Jun 15;21(12):3546-9. doi: 10.1016/j.bmcl.2011.04.137. Epub 2011 May 5.

Authors

Lai-Qiang Ying¹, Bruce P Branchaud

Affiliation

¹ Life Technologies, 29851 Willow Creek Road, Eugene, OR 97402, USA. [email protected]

PMID: 21576021
DOI: 10.1016/j.bmcl.2011.04.137

Abstract

New BODIPY-based pH probes have been designed with excitation and emission wavelengths suitable for fluorescence microscopy and flow cytometry. These pH probes are cell-permeable, selectively label lysosomes, and can be used for noninvasive monitoring of lysosomal pH changes during physiological and pathological processes.

MeSH terms

Acid-Base Equilibrium*
Animals
Boron Compounds / chemical synthesis
Boron Compounds / chemistry*
Cattle
Cell Line
Flow Cytometry
Fluorescent Dyes / chemistry*
Hydrogen-Ion Concentration
Lysosomes / chemistry*
Lysosomes / metabolism*
Microscopy, Confocal
Models, Biological*
Molecular Structure

Substances

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Boron Compounds
Fluorescent Dyes