Abstract
Gene transfer of specific growth factors is suitable for inducing chondrogenic differentiation of -mesenchymal cells to be used for cartilage regeneration. However, extent and quality of repair tissue formation also depend on biomechanical and metabolic influences that can only be studied in vivo. We describe three methods to evaluate viral gene transfer into mesenchymal cells in animal models of articular cartilage defects, e.g., mouse, rat and miniature pig models, focussing on the repair of hyaline cartilage tissue.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / genetics
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Animals
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Cartilage / physiology*
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Cartilage, Articular / injuries
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Cartilage, Articular / pathology
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Cartilage, Articular / virology
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Cell Transplantation / methods*
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Cells, Cultured
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Dependovirus / genetics
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Female
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Gene Transfer Techniques*
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Genes, Reporter
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Genetic Vectors
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Humans
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Intercellular Signaling Peptides and Proteins / genetics
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Knee Injuries / therapy
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Knee Injuries / virology
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Mesenchymal Stem Cell Transplantation
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Mesenchymal Stem Cells / cytology
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Mesenchymal Stem Cells / virology*
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Mice
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Rats
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Rats, Wistar
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Regeneration*
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Swine
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Tissue Scaffolds
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beta-Galactosidase / biosynthesis
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beta-Galactosidase / genetics
Substances
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Intercellular Signaling Peptides and Proteins
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beta-Galactosidase