Abstract
Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis* / drug effects
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Apoptosis* / radiation effects
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Autophagy* / drug effects
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Autophagy* / radiation effects
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Chloroquine / pharmacology
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Embryo, Mammalian / cytology
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Fibroblasts / radiation effects
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Humans
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Image Cytometry / methods*
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / drug effects
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Leukocytes, Mononuclear / metabolism
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Leukocytes, Mononuclear / radiation effects
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Lysosomes / drug effects
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Lysosomes / metabolism
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Lysosomes / radiation effects
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Mice
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Microtubule-Associated Proteins / metabolism
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Signal Transduction / drug effects
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Signal Transduction / radiation effects
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Time Factors
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Ultraviolet Rays
Substances
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Map1lc3b protein, mouse
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Microtubule-Associated Proteins
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Chloroquine