Abstract
Adenosine is able to prevent Ca+(+) influx into activated neutrophils as detected by the specific fluorescent indicator Quin 2. Such an effect is shown in a similar fashion by the calcium entry blocker flunarizine. The binding interaction between flunarizine and the neutrophil membrane as well as the flunarizine-adenosine antagonism are shown by the 1H-NMR technique, thus supporting evidence of a competition between the agents at the same or a nearby site on the cell membrane.
MeSH terms
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Adenosine / metabolism
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Adenosine / pharmacology*
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Aminoquinolines
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Binding Sites
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Calcium / metabolism*
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Calcium Channels / drug effects
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Calcium Channels / metabolism
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Flunarizine / pharmacology
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Fluorescent Dyes
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Humans
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In Vitro Techniques
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Magnetic Resonance Spectroscopy
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology
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Neutrophils / drug effects
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Neutrophils / immunology
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Neutrophils / metabolism*
Substances
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Aminoquinolines
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Calcium Channels
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Fluorescent Dyes
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N-Formylmethionine Leucyl-Phenylalanine
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Adenosine
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Quin2
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Flunarizine
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Calcium