A probe-based method for confirmation of methicillin-resistant Staphylococcus aureus and detection of Panton-Valentine leukocidin and tst virulence genes

Diagn Microbiol Infect Dis. 2011 Aug;70(4):541-3. doi: 10.1016/j.diagmicrobio.2011.04.005. Epub 2011 Jun 11.

Abstract

Probe-based detection of mecA, lukS/F-PV (Panton-Valentine leukocidin), and tst virulence genes in 435 isolates of Staphylococcus aureus had comparable sensitivity and specificity to end-point polymerase chain reaction as a reference standard.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Toxins / genetics*
  • Enterotoxins / genetics*
  • Exotoxins / genetics*
  • Humans
  • Leukocidins / genetics*
  • Methicillin-Resistant Staphylococcus aureus / genetics*
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification*
  • Molecular Diagnostic Techniques / methods*
  • Oligonucleotide Probes / genetics
  • Sensitivity and Specificity
  • Staphylococcal Infections / microbiology*
  • Superantigens / genetics*
  • Virulence Factors / genetics*

Substances

  • Bacterial Toxins
  • Enterotoxins
  • Exotoxins
  • Leukocidins
  • Oligonucleotide Probes
  • Panton-Valentine leukocidin
  • Superantigens
  • Virulence Factors
  • enterotoxin F, Staphylococcal