Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) from culture and paraffin-embedded tissue samples

Ralf M Hagen; Hagen Frickmann; Mandy Elschner; Falk Melzer; Heinrich Neubauer; Yves P Gauthier; Paul Racz; Sven Poppert

doi:10.1016/j.ijmm.2011.04.017

Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) from culture and paraffin-embedded tissue samples

Int J Med Microbiol. 2011 Nov;301(7):585-90. doi: 10.1016/j.ijmm.2011.04.017. Epub 2011 Jun 11.

Authors

Ralf M Hagen¹, Hagen Frickmann, Mandy Elschner, Falk Melzer, Heinrich Neubauer, Yves P Gauthier, Paul Racz, Sven Poppert

Affiliation

¹ Department for Tropical Medicine at the Bernhard Nocht Institute, Bundeswehr Hospital Hamburg, Bernhard-Nocht-Strasse, Germany. [email protected]

PMID: 21658996
DOI: 10.1016/j.ijmm.2011.04.017

Abstract

We evaluated newly developed probes for rapid identification of Burkholderia (B.) pseudomallei and B. mallei and differentiation from B. thailandensis by fluorescence in situ hybridization (FISH). FISH correctly identified 100% of the tested B. pseudomallei (11), B. mallei (11), and B. thailandensis (1) strains, excluded 100% of all tested negative controls (61), and allowed demonstration of B. pseudomallei infection in a paraffin-embedded spleen tissue sample of an experimentally infected mouse.

Publication types

Evaluation Study

MeSH terms

Animals
Bacteriological Techniques / methods*
Burkholderia mallei / genetics
Burkholderia mallei / isolation & purification*
Burkholderia pseudomallei / genetics
Burkholderia pseudomallei / isolation & purification*
Fluorescent Dyes
Humans
In Situ Hybridization, Fluorescence / methods*
Mice
Oligonucleotide Probes / genetics
Pathology / methods*
Sensitivity and Specificity
Spleen / microbiology

Substances

Fluorescent Dyes
Oligonucleotide Probes