Rapid detection of "highly virulent" Group B Streptococcus ST-17 and emerging ST-1 clones by MALDI-TOF mass spectrometry

Marie-Frédérique Lartigue; Markus Kostrzewa; Mazen Salloum; Eve Haguenoer; Geneviève Héry-Arnaud; Anne-Sophie Domelier; Simone Stumpf; Roland Quentin

doi:10.1016/j.mimet.2011.05.017

Rapid detection of "highly virulent" Group B Streptococcus ST-17 and emerging ST-1 clones by MALDI-TOF mass spectrometry

J Microbiol Methods. 2011 Aug;86(2):262-5. doi: 10.1016/j.mimet.2011.05.017. Epub 2011 Jun 2.

Authors

Marie-Frédérique Lartigue¹, Markus Kostrzewa, Mazen Salloum, Eve Haguenoer, Geneviève Héry-Arnaud, Anne-Sophie Domelier, Simone Stumpf, Roland Quentin

Affiliation

¹ Université François-Rabelais de Tours, UFR de Médecine, EA 3854 Bactéries et Risque Materno-foetal, Institut Fédératif de Recherche 136 Agents Transmissibles et Infectiologie, 37032 Tours Cedex, France. [email protected]

PMID: 21663770
DOI: 10.1016/j.mimet.2011.05.017

Abstract

MALDI-TOF MS identified a 6250-Da protein specific to Sequence Type-1 (ST-1) strains and a 7625-Da protein specific to ST-17 strains when used for identification of Group B streptococci. The strains of these STs are major causes of meningitis and late-onset-disease in neonates. This rapid method of identification could thus be valuable in the evaluation of risk of neonatal diseases.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Bacteriological Techniques / methods*
Female
Humans
Infant, Newborn
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Streptococcal Infections / diagnosis*
Streptococcus agalactiae / chemistry
Streptococcus agalactiae / classification*
Streptococcus agalactiae / isolation & purification*