Thiophenyl oxime-derived phosphonates as nano-molar class C beta-lactamase inhibitors reducing MIC of imipenem against Pseudomonas aeruginosa and Acinetobacter baumannii

Bioorg Med Chem Lett. 2011 Jul 15;21(14):4363-5. doi: 10.1016/j.bmcl.2011.04.122. Epub 2011 May 13.

Abstract

The preparation and characterization of a series of thiophenyl oxime phosphonate beta-lactamase inhibitors is described. A number of these analogs were potent and selective inhibitors of class C beta-lactamases from Pseudomonas aeruginosa and Enterobacter cloacae. Compounds 3b and 7 reduced the MIC of imipenem against an AmpC expressing strain of imipenem-resistant P. aeruginosa. A number of the title compounds retained micromolar potency against the class D OXA-40 beta-lactamase from Acinetobacter baumannii and at high concentrations compound 3b was shown to reduce the MIC of imipenem against a highly imipenem-resistant strain of A. baumanii expressing the OXA-40 beta-lactamase. In mice compound 3b exhibited phamacokinetics similar to imipenem.

MeSH terms

  • Acinetobacter baumannii / drug effects*
  • Anti-Bacterial Agents / chemical synthesis
  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacology
  • Drug Resistance, Bacterial
  • Enzyme Inhibitors / chemical synthesis
  • Enzyme Inhibitors / chemistry*
  • Enzyme Inhibitors / pharmacology
  • Imipenem / pharmacology
  • Microbial Sensitivity Tests
  • Organophosphonates / chemical synthesis
  • Organophosphonates / chemistry*
  • Organophosphonates / pharmacology
  • Oximes / chemical synthesis
  • Oximes / chemistry*
  • Oximes / pharmacology
  • Pseudomonas aeruginosa / drug effects*
  • Thiophenes / chemistry
  • beta-Lactamase Inhibitors*
  • beta-Lactamases / metabolism

Substances

  • Anti-Bacterial Agents
  • Enzyme Inhibitors
  • Organophosphonates
  • Oximes
  • Thiophenes
  • beta-Lactamase Inhibitors
  • Imipenem
  • beta-Lactamases