Abstract
E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Improvement of infection with E. canis isolated by this method was detected by real-time PCR.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Benzothiazoles
-
Cell Line
-
Cell Separation / instrumentation*
-
Cell Separation / methods*
-
DNA, Bacterial / analysis
-
Diamines
-
Dog Diseases / microbiology
-
Dogs
-
Ehrlichia canis / genetics
-
Ehrlichia canis / isolation & purification*
-
Ehrlichiosis / microbiology
-
Fluorescent Dyes / metabolism*
-
Glass
-
Humans
-
Organic Chemicals / metabolism*
-
Polymerase Chain Reaction / methods*
-
Quinolines
Substances
-
Benzothiazoles
-
DNA, Bacterial
-
Diamines
-
Fluorescent Dyes
-
Organic Chemicals
-
Quinolines
-
SYBR Green I