Abstract
To determine the normal function of the Coxsackievirus and Adenovirus Receptor (CAR), a protein found in tight junctions and other intercellular complexes, we constructed a mouse line in which the CAR gene could be disrupted at any chosen time point in a broad spectrum of cell types and tissues. All knockouts examined displayed a dilated intestinal tract and atrophy of the exocrine pancreas with appearance of tubular complexes characteristic of acinar-to-ductal metaplasia. The mice also exhibited a complete atrio-ventricular block and abnormal thymopoiesis. These results demonstrate that CAR exerts important functions in the physiology of several organs in vivo.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Atrioventricular Block / genetics
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Atrophy / genetics
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Behavior, Animal / drug effects
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Coxsackie and Adenovirus Receptor-Like Membrane Protein
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Female
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Fertility / drug effects
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Fertility / genetics
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Gene Knockout Techniques
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Gene Silencing* / drug effects
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Intestinal Mucosa / metabolism
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Intestines / drug effects
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Intestines / pathology
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Male
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Mice
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Motor Activity / drug effects
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Motor Activity / genetics
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Pancreas, Exocrine / drug effects
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Pancreas, Exocrine / metabolism
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Pancreas, Exocrine / pathology
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Phenotype*
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Receptors, Virus / deficiency*
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Receptors, Virus / genetics*
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Receptors, Virus / metabolism
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Tamoxifen / pharmacology
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Thymus Gland / cytology
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Thymus Gland / drug effects
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Thymus Gland / metabolism
Substances
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CLMP protein, mouse
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Coxsackie and Adenovirus Receptor-Like Membrane Protein
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Receptors, Virus
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Tamoxifen