Abstract
The herpes simplex virus transactivator Vmw65 assembles into a multicomponent protein-DNA complex along with the octamer binding protein Oct-1. Using affinity chromatography on columns conjugated with purified Vmw65 fusion protein expressed in Escherichia coli, we demonstrate that a cellular factor, distinct from Oct-1, binds to Vmw65 in the absence of target DNA and is necessary for Vmw65-mediated complex assembly with Oct-1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cell Transformation, Viral*
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DNA Probes
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DNA-Binding Proteins / metabolism*
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HeLa Cells / metabolism
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Host Cell Factor C1
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Humans
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Molecular Sequence Data
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Octamer Transcription Factor-1
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Promoter Regions, Genetic
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Simplexvirus / genetics*
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Simplexvirus / metabolism
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Trans-Activators / metabolism*
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Transcription Factors / metabolism*
Substances
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DNA Probes
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DNA-Binding Proteins
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HCFC1 protein, human
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Host Cell Factor C1
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Octamer Transcription Factor-1
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POU2F1 protein, human
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Trans-Activators
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Transcription Factors