Abstract
Respiratory syncytial virus (RSV) G protein deletion mutants replicate effectively in vitro but have not been detected in nature. Subtyping of RSV strains in hospitalized children in South Africa identified G protein PCR amplicons significantly reduced in size in 2 out of 209 clinical specimens screened over 4 years. Sequence analysis revealed subtype B strains lacking nearly the entire G protein ectodomain in one HIV-positive and one HIV-exposed child hospitalized with pneumonia. The association of clinical strains lacking most of the G protein with lower respiratory tract infection in immunocompromised children may have implications for RSV vaccine development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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CD4-Positive T-Lymphocytes / immunology
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Child
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Child, Preschool
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Humans
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Immunocompromised Host
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Infant
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Molecular Sequence Data
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Pneumonia, Viral / immunology
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Pneumonia, Viral / virology*
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Polymerase Chain Reaction
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RNA, Viral / genetics
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Respiratory Syncytial Virus Infections / immunology
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Respiratory Syncytial Virus Infections / virology*
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Respiratory Syncytial Virus, Human / genetics*
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Respiratory Syncytial Virus, Human / growth & development
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Sequence Alignment
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Sequence Deletion*
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South Africa
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Viral Fusion Proteins / chemistry
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Viral Fusion Proteins / genetics*
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Viral Fusion Proteins / immunology
Substances
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G glycoprotein, Respiratory syncytial virus
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RNA, Viral
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Viral Fusion Proteins
Associated data
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GENBANK/HQ711840
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GENBANK/HQ711841
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GENBANK/HQ711842
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GENBANK/JF704213
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GENBANK/JF704214
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GENBANK/JF704215
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GENBANK/JF704216
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GENBANK/JF704217
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GENBANK/JF704218
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GENBANK/JF704219