MRM assay for quantitation of complement components in human blood plasma - a feasibility study on multiple sclerosis

J Proteomics. 2011 Dec 10;75(1):211-20. doi: 10.1016/j.jprot.2011.05.042. Epub 2011 Jun 15.

Abstract

As a proof-of-principle study, a multiple reaction monitoring (MRM) assay was developed for quantitation of proteotypic peptides, representing seven plasma proteins associated with inflammation (complement components and C-reactive protein). The assay development and the sample analysis were performed on a linear ion trap mass spectrometer. We were able to quantify 5 of the 7 target proteins in depleted plasma digests with reasonable reproducibility over a 2 orders of magnitude linear range (RSD≤25%). The assay panel was utilized for the analysis of a small multiple sclerosis sample cohort with 10 diseased and 8 control patients.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • C-Reactive Protein / analysis*
  • C-Reactive Protein / chemistry
  • C-Reactive Protein / metabolism
  • Case-Control Studies
  • Complement System Proteins / analysis*
  • Complement System Proteins / chemistry
  • Complement System Proteins / metabolism
  • Feasibility Studies
  • Humans
  • Inflammation / metabolism
  • Inflammation / pathology
  • Mass Spectrometry / methods
  • Multiple Sclerosis / diagnosis*
  • Multiple Sclerosis / metabolism
  • Peptides / analysis
  • Peptides / blood
  • Peptides / chemistry
  • Plasma / metabolism*
  • Proteomics / methods*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Peptides
  • Complement System Proteins
  • C-Reactive Protein