Abstract
Plasmid pJRDC800-1, containing the cyc gene encoding cytochrome c3 from Desulfovibrio vulgaris subsp. vulgaris Hildenborough, was transferred by conjugation from Escherichia coli DH5 alpha to Desulfovibrio desulfuricans G200. The G200 strain produced an acidic cytochrome c3 (pI = 5.8), which could be readily separated from the Hildenborough cytochrome c3 (pI = 10.5). The latter was indistinguishable from cytochrome c3 produced by D. vulgaris subsp. vulgaris Hildenborough with respect to a number of chemical and physical criteria.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Conjugation, Genetic*
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Cytochrome c Group / genetics*
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Cytochrome c Group / isolation & purification
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Cytochrome c Group / metabolism
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Desulfovibrio / genetics*
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Desulfovibrio / metabolism
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Genotype
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Kinetics
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Magnetic Resonance Spectroscopy
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Transfection
Substances
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Cytochrome c Group
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Recombinant Proteins
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cytochrome c(3)