Fully automated one-pot radiosynthesis of O-(2-[18F]fluoroethyl)-L-tyrosine on the TracerLab FX(FN) module

Nucl Med Biol. 2011 Jul;38(5):645-51. doi: 10.1016/j.nucmedbio.2011.01.001. Epub 2011 Mar 3.

Abstract

Introduction: An efficient fully automated method for the radiosynthesis of enantiomerically pure O-(2-[(18)F]fluoroethyl)-L-tyrosine ([(18)F]FET) using the GE TracerLab FX(FN) synthesis module via the O-(2-tosyloxyethyl)-N-trityl-L-tyrosine tert-butylester precursor has been developed.

Methods: The radiolabelling of [(18)F]FET involved a classical [(18)F]fluoride nucleophilic substitution performed in acetonitrile using potassium carbonate and Kryptofix 222, followed by acid hydrolysis using 2N hydrochloric acid.

Results: [(18)F]FET was produced in 35±5% (n=22) yield non-decay-corrected (55±5% decay-corrected) and with radiochemical and enantiomeric purity of >99% with a specific activity of >90 GBq/μmol after 63 min of radiosynthesis including HPLC purification and formulation.

Conclusion: The automated radiosynthesis provides high and reproducible yields suitable for routine clinical use.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Automation
  • Indicators and Reagents / chemistry
  • Laboratories
  • Quality Control
  • Radiochemistry / instrumentation*
  • Radiochemistry / standards
  • Reproducibility of Results
  • Tyrosine / analogs & derivatives*
  • Tyrosine / chemical synthesis
  • Tyrosine / chemistry

Substances

  • Indicators and Reagents
  • O-(2-fluoroethyl)tyrosine
  • Tyrosine