IL-18 induces PD-1-dependent immunosuppression in cancer

Cancer Res. 2011 Aug 15;71(16):5393-9. doi: 10.1158/0008-5472.CAN-11-0993. Epub 2011 Jul 1.

Abstract

Immunosuppressive cytokines subvert innate and adaptive immune responses during cancer progression. The inflammatory cytokine interleukin-18 (IL-18) is known to accumulate in cancer patients, but its pathophysiological role remains unclear. In this study, we show that low levels of circulating IL-18, either exogenous or tumor derived, act to suppress the NK cell arm of tumor immunosurveillance. IL-18 produced by tumor cells promotes the development of NK-controlled metastases in a PD-1-dependent manner. Accordingly, PD-1 is expressed by activated mature NK cells in lymphoid organs of tumor bearers and is upregulated by IL-18. RNAi-mediated knockdown of IL-18 in tumors, or its systemic depletion by IL-18-binding protein, are sufficient to stimulate NK cell-dependent immunosurveillance in various tumor models. Together, these results define IL-18 as an immunosuppressive cytokine in cancer. Our findings suggest novel clinical implementations of anti-PD-1 antibodies in human malignancies that produce IL-18.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Surface / immunology
  • Antigens, Surface / physiology*
  • Apoptosis Regulatory Proteins / immunology
  • Apoptosis Regulatory Proteins / physiology*
  • Autoantibodies / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Immune Tolerance
  • Interleukin-18 / physiology*
  • Killer Cells, Natural / immunology
  • Killer Cells, Natural / pathology
  • Melanoma, Experimental / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Neoplasm Metastasis / immunology
  • Programmed Cell Death 1 Receptor

Substances

  • Antigens, Surface
  • Apoptosis Regulatory Proteins
  • Autoantibodies
  • Interleukin-18
  • Pdcd1 protein, mouse
  • Programmed Cell Death 1 Receptor